DMOG (Dimethyloxallyl Glycine)

DMOG zeigt im mikrosomalen System nur eine schwache Aktivität, unterdrückt aber effizient die Hydroxyprolin-Synthese in intakten Zellen. [1] Diese Verbindung reduziert die FGF-2-induzierte Proliferation und Cyclin-A-Expression durch Hemmung der Prolylhydroxylase-Aktivität in HPASMC. [3]

DMOG hemmt die endogene HIF-Inaktivierung und induziert die Angiogenese in ischämischen Skelettmuskeln von Mäusen. [2] Die Hochregulierung von Hypoxie-induzierbarem Faktor-1α durch diese Verbindung verstärkt die kardioprotektiven Effekte des ischämischen Postconditionings bei hyperlipidämischen Ratten. [4]

• Zelllinien

  VSMC cells

• Konzentrationen

  ~100 μM

• Inkubationszeit

  72-96 hours

• Methode

  To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

• Tiermodelle

  Mice (C57Bl6)

• Dosierungen

  8 mg in 0.5 ml saline

• Verabreichung

  i.p.