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Torin 1 mTOR Inhibitor

Name: Torin 1
Brand: Selleck Chemicals
SKU: S2827
Rating: 5 (247 reviews)

Kat.-Nr.S2827

Torin 1 ist ein potenter Inhibitor von mTORC1/2 mit einer IC50 von 2 nM/10 nM in zellfreien Assays; zeigt eine 1000-fache Selektivität für mTOR im Vergleich zu PI3K.

Torin 1 mTOR Inhibitor Chemical Structure

Chemische Struktur

Molekulargewicht: 607.62

Springe zu

Qualitätskontrolle

Charge: Reinheit: 99.07%

99.07

Verwandte Ziele	PI3K Akt GSK-3 ATM/ATR DNA-PK AMPK PDPK1 PTEN PP2A PDK
Weitere mTOR Inhibitoren	Torin 2 AZD8055 Ridaforolimus (Deforolimus, MK-8669) Sapanisertib (MLN0128, INK-128) Torkinib (PP242) MHY1485 Vistusertib (AZD2014) KU-0063794 OSI-027 WYE-354

Zellkultur, Behandlung & Arbeitskonzentration

Zelllinien	Assay-Typ	Aktivitätsbeschreibung	PMID
ETK-1	Growth Inhibition Assay	IC50=0.000306 μM	SANGER
OCUB-M	Growth Inhibition Assay	IC50=0.00194 μM	SANGER
DOHH-2	Growth Inhibition Assay	IC50=0.00225 μM	SANGER
NOS-1	Growth Inhibition Assay	IC50=0.00242 μM	SANGER
L-363	Growth Inhibition Assay	IC50=0.00284 μM	SANGER
RL95-2	Growth Inhibition Assay	IC50=0.0032 μM	SANGER
BL-70	Growth Inhibition Assay	IC50=0.00337 μM	SANGER
TGBC1TKB	Growth Inhibition Assay	IC50=0.00379 μM	SANGER
697	Growth Inhibition Assay	IC50=0.00634 μM	SANGER
RPMI-8226	Growth Inhibition Assay	IC50=0.00651 μM	SANGER
BL-41	Growth Inhibition Assay	IC50=0.00696 μM	SANGER
MRK-nu-1	Growth Inhibition Assay	IC50=0.00707 μM	SANGER
EW-1	Growth Inhibition Assay	IC50=0.00749 μM	SANGER
EVSA-T	Growth Inhibition Assay	IC50=0.00892 μM	SANGER
HUTU-80	Growth Inhibition Assay	IC50=0.00929 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay	IC50=0.00984 μM	SANGER
GCIY	Growth Inhibition Assay	IC50=0.01007 μM	SANGER
ES6	Growth Inhibition Assay	IC50=0.01083 μM	SANGER
NALM-6	Growth Inhibition Assay	IC50=0.01102 μM	SANGER
LB996-RCC	Growth Inhibition Assay	IC50=0.01137 μM	SANGER
EW-16	Growth Inhibition Assay	IC50=0.01192 μM	SANGER
KALS-1	Growth Inhibition Assay	IC50=0.01198 μM	SANGER
OPM-2	Growth Inhibition Assay	IC50=0.0129 μM	SANGER
KU812	Growth Inhibition Assay	IC50=0.01296 μM	SANGER
A4-Fuk	Growth Inhibition Assay	IC50=0.01319 μM	SANGER
KGN	Growth Inhibition Assay	IC50=0.01359 μM	SANGER
KS-1	Growth Inhibition Assay	IC50=0.01392 μM	SANGER
no-11	Growth Inhibition Assay	IC50=0.01395 μM	SANGER
PSN1	Growth Inhibition Assay	IC50=0.01409 μM	SANGER
SK-LMS-1	Growth Inhibition Assay	IC50=0.01413 μM	SANGER
LS-411N	Growth Inhibition Assay	IC50=0.01439 μM	SANGER
HT-144	Growth Inhibition Assay	IC50=0.01451 μM	SANGER
ACN	Growth Inhibition Assay	IC50=0.015 μM	SANGER
RKO	Growth Inhibition Assay	IC50=0.01533 μM	SANGER
CMK	Growth Inhibition Assay	IC50=0.01543 μM	SANGER
GB-1	Growth Inhibition Assay	IC50=0.01553 μM	SANGER
KE-37	Growth Inhibition Assay	IC50=0.01573 μM	SANGER
no-10	Growth Inhibition Assay	IC50=0.01584 μM	SANGER
MOLT-16	Growth Inhibition Assay	IC50=0.01622 μM	SANGER
HEL	Growth Inhibition Assay	IC50=0.0164 μM	SANGER
NCI-H1355	Growth Inhibition Assay	IC50=0.01646 μM	SANGER
IST-MEL1	Growth Inhibition Assay	IC50=0.01774 μM	SANGER
MHH-PREB-1	Growth Inhibition Assay	IC50=0.01818 μM	SANGER
ES8	Growth Inhibition Assay	IC50=0.01844 μM	SANGER
LC-2-ad	Growth Inhibition Assay	IC50=0.02137 μM	SANGER
LAMA-84	Growth Inhibition Assay	IC50=0.02167 μM	SANGER
BV-173	Growth Inhibition Assay	IC50=0.02203 μM	SANGER
SW872	Growth Inhibition Assay	IC50=0.02247 μM	SANGER
SK-UT-1	Growth Inhibition Assay	IC50=0.02261 μM	SANGER
OS-RC-2	Growth Inhibition Assay	IC50=0.02323 μM	SANGER
NCI-H747	Growth Inhibition Assay	IC50=0.0233 μM	SANGER
MZ2-MEL	Growth Inhibition Assay	IC50=0.02337 μM	SANGER
TE-11	Growth Inhibition Assay	IC50=0.02372 μM	SANGER
KURAMOCHI	Growth Inhibition Assay	IC50=0.02414 μM	SANGER
HD-MY-Z	Growth Inhibition Assay	IC50=0.02426 μM	SANGER
GI-1	Growth Inhibition Assay	IC50=0.02469 μM	SANGER
LB1047-RCC	Growth Inhibition Assay	IC50=0.02488 μM	SANGER
TE-12	Growth Inhibition Assay	IC50=0.0255 μM	SANGER
MZ7-mel	Growth Inhibition Assay	IC50=0.02609 μM	SANGER
MMAC-SF	Growth Inhibition Assay	IC50=0.02633 μM	SANGER
A101D	Growth Inhibition Assay	IC50=0.0265 μM	SANGER
GR-ST	Growth Inhibition Assay	IC50=0.02655 μM	SANGER
MC116	Growth Inhibition Assay	IC50=0.0268 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay	IC50=0.0275 μM	SANGER
ATN-1	Growth Inhibition Assay	IC50=0.02789 μM	SANGER
MFH-ino	Growth Inhibition Assay	IC50=0.02812 μM	SANGER
DEL	Growth Inhibition Assay	IC50=0.02815 μM	SANGER
OCI-AML2	Growth Inhibition Assay	IC50=0.02871 μM	SANGER
LXF-289	Growth Inhibition Assay	IC50=0.02872 μM	SANGER
IA-LM	Growth Inhibition Assay	IC50=0.02917 μM	SANGER
EoL-1-cell	Growth Inhibition Assay	IC50=0.02996 μM	SANGER
OVCAR-4	Growth Inhibition Assay	IC50=0.03094 μM	SANGER
ES1	Growth Inhibition Assay	IC50=0.03097 μM	SANGER
CESS	Growth Inhibition Assay	IC50=0.03099 μM	SANGER
GAK	Growth Inhibition Assay	IC50=0.03186 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay	IC50=0.03226 μM	SANGER
TE-10	Growth Inhibition Assay	IC50=0.03311 μM	SANGER
CCRF-CEM	Growth Inhibition Assay	IC50=0.03371 μM	SANGER
NMC-G1	Growth Inhibition Assay	IC50=0.03448 μM	SANGER
HOP-62	Growth Inhibition Assay	IC50=0.03556 μM	SANGER
SH-4	Growth Inhibition Assay	IC50=0.03582 μM	SANGER
NCI-H1648	Growth Inhibition Assay	IC50=0.03622 μM	SANGER
EB-3	Growth Inhibition Assay	IC50=0.03643 μM	SANGER
BB65-RCC	Growth Inhibition Assay	IC50=0.03691 μM	SANGER
BE-13	Growth Inhibition Assay	IC50=0.03759 μM	SANGER
CP67-MEL	Growth Inhibition Assay	IC50=0.03759 μM	SANGER
SW954	Growth Inhibition Assay	IC50=0.0379 μM	SANGER
TE-5	Growth Inhibition Assay	IC50=0.03854 μM	SANGER
HCE-4	Growth Inhibition Assay	IC50=0.03855 μM	SANGER
LC-1F	Growth Inhibition Assay	IC50=0.03989 μM	SANGER
TK10	Growth Inhibition Assay	IC50=0.04019 μM	SANGER
SJSA-1	Growth Inhibition Assay	IC50=0.04188 μM	SANGER
Daudi	Growth Inhibition Assay	IC50=0.04225 μM	SANGER
SW982	Growth Inhibition Assay	IC50=0.04246 μM	SANGER
SF126	Growth Inhibition Assay	IC50=0.04361 μM	SANGER
TE-15	Growth Inhibition Assay	IC50=0.04435 μM	SANGER
COLO-684	Growth Inhibition Assay	IC50=0.04561 μM	SANGER
8-MG-BA	Growth Inhibition Assay	IC50=0.04629 μM	SANGER
NB13	Growth Inhibition Assay	IC50=0.04776 μM	SANGER
HCC1599	Growth Inhibition Assay	IC50=0.04879 μM	SANGER
SK-PN-DW	Growth Inhibition Assay	IC50=0.04949 μM	SANGER
ALL-PO	Growth Inhibition Assay	IC50=0.04956 μM	SANGER
NB6	Growth Inhibition Assay	IC50=0.05271 μM	SANGER
IST-SL2	Growth Inhibition Assay	IC50=0.05362 μM	SANGER
BB30-HNC	Growth Inhibition Assay	IC50=0.0573 μM	SANGER
SW962	Growth Inhibition Assay	IC50=0.05763 μM	SANGER
TGBC24TKB	Growth Inhibition Assay	IC50=0.05799 μM	SANGER
KINGS-1	Growth Inhibition Assay	IC50=0.05803 μM	SANGER
HC-1	Growth Inhibition Assay	IC50=0.05827 μM	SANGER
RS4-11	Growth Inhibition Assay	IC50=0.05938 μM	SANGER
LS-123	Growth Inhibition Assay	IC50=0.05942 μM	SANGER
A3-KAW	Growth Inhibition Assay	IC50=0.06225 μM	SANGER
SNB75	Growth Inhibition Assay	IC50=0.06341 μM	SANGER
SR	Growth Inhibition Assay	IC50=0.06374 μM	SANGER
LOUCY	Growth Inhibition Assay	IC50=0.06394 μM	SANGER
K5	Growth Inhibition Assay	IC50=0.06403 μM	SANGER
Becker	Growth Inhibition Assay	IC50=0.0642 μM	SANGER
CTV-1	Growth Inhibition Assay	IC50=0.06548 μM	SANGER
KLE	Growth Inhibition Assay	IC50=0.06584 μM	SANGER
MZ1-PC	Growth Inhibition Assay	IC50=0.06598 μM	SANGER
MOLT-4	Growth Inhibition Assay	IC50=0.06605 μM	SANGER
MLMA	Growth Inhibition Assay	IC50=0.0678 μM	SANGER
A388	Growth Inhibition Assay	IC50=0.06893 μM	SANGER
MV-4-11	Growth Inhibition Assay	IC50=0.06995 μM	SANGER
DSH1	Growth Inhibition Assay	IC50=0.07173 μM	SANGER
MONO-MAC-6	Growth Inhibition Assay	IC50=0.07212 μM	SANGER
ARH-77	Growth Inhibition Assay	IC50=0.07278 μM	SANGER
LB771-HNC	Growth Inhibition Assay	IC50=0.07549 μM	SANGER
PF-382	Growth Inhibition Assay	IC50=0.07569 μM	SANGER
KM12	Growth Inhibition Assay	IC50=0.0764 μM	SANGER
HAL-01	Growth Inhibition Assay	IC50=0.07658 μM	SANGER
RPMI-8402	Growth Inhibition Assay	IC50=0.07789 μM	SANGER
OMC-1	Growth Inhibition Assay	IC50=0.07804 μM	SANGER
HT	Growth Inhibition Assay	IC50=0.08 μM	SANGER
QIMR-WIL	Growth Inhibition Assay	IC50=0.08055 μM	SANGER
KNS-42	Growth Inhibition Assay	IC50=0.08203 μM	SANGER
BC-3	Growth Inhibition Assay	IC50=0.08204 μM	SANGER
ES4	Growth Inhibition Assay	IC50=0.08296 μM	SANGER
NCI-H23	Growth Inhibition Assay	IC50=0.0844 μM	SANGER
LB2518-MEL	Growth Inhibition Assay	IC50=0.08696 μM	SANGER
LC4-1	Growth Inhibition Assay	IC50=0.08738 μM	SANGER
SIG-M5	Growth Inhibition Assay	IC50=0.08904 μM	SANGER
LP-1	Growth Inhibition Assay	IC50=0.08938 μM	SANGER
TE-8	Growth Inhibition Assay	IC50=0.09016 μM	SANGER
K-562	Growth Inhibition Assay	IC50=0.0912 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay	IC50=0.09153 μM	SANGER
DU-4475	Growth Inhibition Assay	IC50=0.09165 μM	SANGER
RXF393	Growth Inhibition Assay	IC50=0.09462 μM	SANGER
NB5	Growth Inhibition Assay	IC50=0.09625 μM	SANGER
A704	Growth Inhibition Assay	IC50=0.09741 μM	SANGER
SF268	Growth Inhibition Assay	IC50=0.09825 μM	SANGER
UACC-257	Growth Inhibition Assay	IC50=0.09833 μM	SANGER
L-428	Growth Inhibition Assay	IC50=0.1032 μM	SANGER
ST486	Growth Inhibition Assay	IC50=0.10606 μM	SANGER
SK-MM-2	Growth Inhibition Assay	IC50=0.10709 μM	SANGER
COR-L279	Growth Inhibition Assay	IC50=0.10832 μM	SANGER
JiyoyeP-2003	Growth Inhibition Assay	IC50=0.10843 μM	SANGER
GT3TKB	Growth Inhibition Assay	IC50=0.10918 μM	SANGER
HCE-T	Growth Inhibition Assay	IC50=0.1094 μM	SANGER
SBC-1	Growth Inhibition Assay	IC50=0.11119 μM	SANGER
KNS-81-FD	Growth Inhibition Assay	IC50=0.11273 μM	SANGER
NCI-H2126	Growth Inhibition Assay	IC50=0.11394 μM	SANGER
EM-2	Growth Inhibition Assay	IC50=0.11529 μM	SANGER
JAR	Growth Inhibition Assay	IC50=0.11598 μM	SANGER
NB17	Growth Inhibition Assay	IC50=0.11652 μM	SANGER
LB647-SCLC	Growth Inhibition Assay	IC50=0.11705 μM	SANGER
CAL-148	Growth Inhibition Assay	IC50=0.118 μM	SANGER
K052	Growth Inhibition Assay	IC50=0.11851 μM	SANGER
KARPAS-299	Growth Inhibition Assay	IC50=0.12233 μM	SANGER
KARPAS-422	Growth Inhibition Assay	IC50=0.12432 μM	SANGER
LOXIMVI	Growth Inhibition Assay	IC50=0.12515 μM	SANGER
SCC-3	Growth Inhibition Assay	IC50=0.12851 μM	SANGER
LU-139	Growth Inhibition Assay	IC50=0.12975 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay	IC50=0.13086 μM	SANGER
ONS-76	Growth Inhibition Assay	IC50=0.1313 μM	SANGER
U-266	Growth Inhibition Assay	IC50=0.13245 μM	SANGER
HH	Growth Inhibition Assay	IC50=0.13535 μM	SANGER
BB49-HNC	Growth Inhibition Assay	IC50=0.13587 μM	SANGER
NCI-SNU-5	Growth Inhibition Assay	IC50=0.13596 μM	SANGER
C2BBe1	Growth Inhibition Assay	IC50=0.13746 μM	SANGER
ML-2	Growth Inhibition Assay	IC50=0.13953 μM	SANGER
SCH	Growth Inhibition Assay	IC50=0.13974 μM	SANGER
NCI-H82	Growth Inhibition Assay	IC50=0.14104 μM	SANGER
MDA-MB-134-VI	Growth Inhibition Assay	IC50=0.14252 μM	SANGER
SK-N-DZ	Growth Inhibition Assay	IC50=0.14719 μM	SANGER
HL-60	Growth Inhibition Assay	IC50=0.1492 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay	IC50=0.15539 μM	SANGER
LB831-BLC	Growth Inhibition Assay	IC50=0.16076 μM	SANGER
SK-NEP-1	Growth Inhibition Assay	IC50=0.16101 μM	SANGER
IMR-5	Growth Inhibition Assay	IC50=0.16483 μM	SANGER
Calu-6	Growth Inhibition Assay	IC50=0.16537 μM	SANGER
NCI-H1882	Growth Inhibition Assay	IC50=0.16728 μM	SANGER
NCI-H446	Growth Inhibition Assay	IC50=0.16891 μM	SANGER
GI-ME-N	Growth Inhibition Assay	IC50=0.1802 μM	SANGER
MPP-89	Growth Inhibition Assay	IC50=0.18121 μM	SANGER
CGTH-W-1	Growth Inhibition Assay	IC50=0.18436 μM	SANGER
MEG-01	Growth Inhibition Assay	IC50=0.18628 μM	SANGER
NCI-H748	Growth Inhibition Assay	IC50=0.18911 μM	SANGER
COLO-829	Growth Inhibition Assay	IC50=0.20673 μM	SANGER
GOTO	Growth Inhibition Assay	IC50=0.2073 μM	SANGER
EW-3	Growth Inhibition Assay	IC50=0.21718 μM	SANGER
KY821	Growth Inhibition Assay	IC50=0.22045 μM	SANGER
CTB-1	Growth Inhibition Assay	IC50=0.22228 μM	SANGER
TALL-1	Growth Inhibition Assay	IC50=0.22921 μM	SANGER
NCI-H209	Growth Inhibition Assay	IC50=0.22962 μM	SANGER
IST-MES1	Growth Inhibition Assay	IC50=0.2351 μM	SANGER
DMS-114	Growth Inhibition Assay	IC50=0.23993 μM	SANGER
SNU-C2B	Growth Inhibition Assay	IC50=0.24316 μM	SANGER
DG-75	Growth Inhibition Assay	IC50=0.26195 μM	SANGER
D-336MG	Growth Inhibition Assay	IC50=0.26557 μM	SANGER
KMOE-2	Growth Inhibition Assay	IC50=0.27538 μM	SANGER
NB1	Growth Inhibition Assay	IC50=0.27926 μM	SANGER
RH-1	Growth Inhibition Assay	IC50=0.27989 μM	SANGER
TE-1	Growth Inhibition Assay	IC50=0.28479 μM	SANGER
RPMI-6666	Growth Inhibition Assay	IC50=0.2884 μM	SANGER
TE-9	Growth Inhibition Assay	IC50=0.28985 μM	SANGER
BT-474	Growth Inhibition Assay	IC50=0.29334 μM	SANGER
COLO-668	Growth Inhibition Assay	IC50=0.29429 μM	SANGER
NCI-H1581	Growth Inhibition Assay	IC50=0.29689 μM	SANGER
MN-60	Growth Inhibition Assay	IC50=0.29905 μM	SANGER
L-540	Growth Inhibition Assay	IC50=0.30081 μM	SANGER
MOLT-13	Growth Inhibition Assay	IC50=0.30183 μM	SANGER
NCI-SNU-16	Growth Inhibition Assay	IC50=0.30205 μM	SANGER
NCI-H64	Growth Inhibition Assay	IC50=0.30331 μM	SANGER
CP66-MEL	Growth Inhibition Assay	IC50=0.31415 μM	SANGER
NB69	Growth Inhibition Assay	IC50=0.32188 μM	SANGER
NCI-H2196	Growth Inhibition Assay	IC50=0.33036 μM	SANGER
MHH-CALL-2	Growth Inhibition Assay	IC50=0.33468 μM	SANGER
HCC2157	Growth Inhibition Assay	IC50=0.33954 μM	SANGER
LS-1034	Growth Inhibition Assay	IC50=0.36388 μM	SANGER
BOKU	Growth Inhibition Assay	IC50=0.37199 μM	SANGER
GDM-1	Growth Inhibition Assay	IC50=0.37234 μM	SANGER
TGW	Growth Inhibition Assay	IC50=0.37453 μM	SANGER
SNU-C1	Growth Inhibition Assay	IC50=0.38215 μM	SANGER
LS-513	Growth Inhibition Assay	IC50=0.39071 μM	SANGER
NOMO-1	Growth Inhibition Assay	IC50=0.39971 μM	SANGER
MS-1	Growth Inhibition Assay	IC50=0.40177 μM	SANGER
EKVX	Growth Inhibition Assay	IC50=0.44461 μM	SANGER
SK-MEL-2	Growth Inhibition Assay	IC50=0.464 μM	SANGER
HCC1187	Growth Inhibition Assay	IC50=0.46768 μM	SANGER
HCC2218	Growth Inhibition Assay	IC50=0.47227 μM	SANGER
NCI-H187	Growth Inhibition Assay	IC50=0.4784 μM	SANGER
DB	Growth Inhibition Assay	IC50=0.50055 μM	SANGER
NCI-H69	Growth Inhibition Assay	IC50=0.50247 μM	SANGER
NCI-H1694	Growth Inhibition Assay	IC50=0.50697 μM	SANGER
CA46	Growth Inhibition Assay	IC50=0.50917 μM	SANGER
EHEB	Growth Inhibition Assay	IC50=0.51193 μM	SANGER
NCCIT	Growth Inhibition Assay	IC50=0.54321 μM	SANGER
NCI-H720	Growth Inhibition Assay	IC50=0.54962 μM	SANGER
LU-134-A	Growth Inhibition Assay	IC50=0.55296 μM	SANGER
MC-CAR	Growth Inhibition Assay	IC50=0.55431 μM	SANGER
NEC8	Growth Inhibition Assay	IC50=0.56172 μM	SANGER
NCI-H524	Growth Inhibition Assay	IC50=0.58748 μM	SANGER
NCI-H1963	Growth Inhibition Assay	IC50=0.6258 μM	SANGER
SU-DHL-1	Growth Inhibition Assay	IC50=0.64526 μM	SANGER
NCI-H1522	Growth Inhibition Assay	IC50=0.64883 μM	SANGER
NCI-H1155	Growth Inhibition Assay	IC50=0.6535 μM	SANGER
D-263MG	Growth Inhibition Assay	IC50=0.66101 μM	SANGER
TE-6	Growth Inhibition Assay	IC50=0.68104 μM	SANGER
NCI-H1417	Growth Inhibition Assay	IC50=0.69711 μM	SANGER
NCI-H510A	Growth Inhibition Assay	IC50=0.77236 μM	SANGER
KARPAS-45	Growth Inhibition Assay	IC50=0.78235 μM	SANGER
IST-SL1	Growth Inhibition Assay	IC50=0.85825 μM	SANGER
COLO-824	Growth Inhibition Assay	IC50=0.87835 μM	SANGER
NCI-H526	Growth Inhibition Assay	IC50=0.90958 μM	SANGER
NCI-H719	Growth Inhibition Assay	IC50=0.96657 μM	SANGER
SF539	Growth Inhibition Assay	IC50=0.98396 μM	SANGER
NKM-1	Growth Inhibition Assay	IC50=0.98663 μM	SANGER
A253	Growth Inhibition Assay	IC50=0.98885 μM	SANGER
TE-441-T	Growth Inhibition Assay	IC50=1.0435 μM	SANGER
COR-L88	Growth Inhibition Assay	IC50=1.11354 μM	SANGER
CPC-N	Growth Inhibition Assay	IC50=1.15888 μM	SANGER
NCI-H2107	Growth Inhibition Assay	IC50=1.17261 μM	SANGER
ECC4	Growth Inhibition Assay	IC50=1.18693 μM	SANGER
NCI-H2141	Growth Inhibition Assay	IC50=1.19299 μM	SANGER
NCI-H1770	Growth Inhibition Assay	IC50=1.32928 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay	IC50=1.3304 μM	SANGER
LU-165	Growth Inhibition Assay	IC50=1.45467 μM	SANGER
Raji	Growth Inhibition Assay	IC50=1.47259 μM	SANGER
EW-24	Growth Inhibition Assay	IC50=1.54619 μM	SANGER
KP-N-RT-BM-1	Growth Inhibition Assay	IC50=1.56551 μM	SANGER
SK-MEL-1	Growth Inhibition Assay	IC50=1.64877 μM	SANGER
SCLC-21H	Growth Inhibition Assay	IC50=1.66651 μM	SANGER
SIMA	Growth Inhibition Assay	IC50=1.68233 μM	SANGER
NCI-H1304	Growth Inhibition Assay	IC50=1.70765 μM	SANGER
REH	Growth Inhibition Assay	IC50=1.71078 μM	SANGER
KP-N-YS	Growth Inhibition Assay	IC50=1.93551 μM	SANGER
DMS-153	Growth Inhibition Assay	IC50=1.9759 μM	SANGER
NB10	Growth Inhibition Assay	IC50=2.20878 μM	SANGER
NCI-H345	Growth Inhibition Assay	IC50=2.61905 μM	SANGER
SK-N-FI	Growth Inhibition Assay	IC50=2.75381 μM	SANGER
NCI-H1092	Growth Inhibition Assay	IC50=2.95394 μM	SANGER
KM-H2	Growth Inhibition Assay	IC50=3.65396 μM	SANGER
KG-1	Growth Inhibition Assay	IC50=3.71688 μM	SANGER
D-283MED	Growth Inhibition Assay	IC50=3.7587 μM	SANGER
LU-65	Growth Inhibition Assay	IC50=4.11485 μM	SANGER
MSTO-211H	Growth Inhibition Assay	IC50=4.18898 μM	SANGER
NCI-H2081	Growth Inhibition Assay	IC50=4.29174 μM	SANGER
D-247MG	Growth Inhibition Assay	IC50=4.43191 μM	SANGER
P31-FUJ	Growth Inhibition Assay	IC50=4.45019 μM	SANGER
DMS-79	Growth Inhibition Assay	IC50=4.93183 μM	SANGER
KASUMI-1	Growth Inhibition Assay	IC50=4.97509 μM	SANGER
JVM-3	Growth Inhibition Assay	IC50=5.25014 μM	SANGER
LAN-6	Growth Inhibition Assay	IC50=5.51736 μM	SANGER
DJM-1	Growth Inhibition Assay	IC50=5.56029 μM	SANGER
JVM-2	Growth Inhibition Assay	IC50=5.67092 μM	SANGER
U-87-MG	Growth Inhibition Assay	IC50=5.89964 μM	SANGER
SHP-77	Growth Inhibition Assay	IC50=5.94928 μM	SANGER
RPMI-8866	Growth Inhibition Assay	IC50=6.27013 μM	SANGER
ES7	Growth Inhibition Assay	IC50=6.39924 μM	SANGER
WSU-NHL	Growth Inhibition Assay	IC50=6.51609 μM	SANGER
CAS-1	Growth Inhibition Assay	IC50=7.09749 μM	SANGER
MHH-NB-11	Growth Inhibition Assay	IC50=8.23152 μM	SANGER
ES5	Growth Inhibition Assay	IC50=9.52286 μM	SANGER
HDLM-2	Growth Inhibition Assay	IC50=9.95902 μM	SANGER
NCI-H2171	Growth Inhibition Assay	IC50=10.5927 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay	IC50=11.3718 μM	SANGER
NCI-H1395	Growth Inhibition Assay	IC50=12.2699 μM	SANGER
NCI-H1436	Growth Inhibition Assay	IC50=13.3195 μM	SANGER
RCC10RGB	Growth Inhibition Assay	IC50=13.3221 μM	SANGER
SW684	Growth Inhibition Assay	IC50=13.9797 μM	SANGER
NCI-H1650	Growth Inhibition Assay	IC50=15.0447 μM	SANGER
EW-13	Growth Inhibition Assay	IC50=15.2021 μM	SANGER
EC-GI-10	Growth Inhibition Assay	IC50=15.8644 μM	SANGER
CHP-126	Growth Inhibition Assay	IC50=17.1918 μM	SANGER
NCI-H226	Growth Inhibition Assay	IC50=18.0403 μM	SANGER
NCI-H1838	Growth Inhibition Assay	IC50=20.1848 μM	SANGER
EW-11	Growth Inhibition Assay	IC50=24.2718 μM	SANGER
EB2	Growth Inhibition Assay	IC50=31.5318 μM	SANGER
D-502MG	Growth Inhibition Assay	IC50=43.9229 μM	SANGER
U-698-M	Growth Inhibition Assay	IC50=48.8671 μM	SANGER
ES3	Growth Inhibition Assay	IC50=49.3893 μM	SANGER
PC3	Function assay	Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM.	21322566
TC32	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells	29435139
A673	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
BT-37	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
OHS-50	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
Rh41	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells	29435139
SJ-GBM2	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
SK-N-MC	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells	29435139
TC32	qHTS assay	qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells	29435139
Klicken Sie hier, um weitere experimentelle Daten zu Zelllinien anzuzeigen

Chemische Informationen, Lagerung & Stabilität

Molekulargewicht	607.62	Formel	C₃₅H₂₈F₃N₅O₂	Lagerung (Ab dem Eingangsdatum)	3 Jahre-20°CPulver
CAS-Nr.	1222998-36-8	SDF herunterladen		Lagerung von Stammlösungen	1 Jahr-80°Cin Lösungsmittel 1 Monat-20°Cin Lösungsmittel
Synonyme	N/A			Smiles	CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

Löslichkeit

In vitro
Charge:

DMSO : 3 mg/mL (4.93 mM)
(Feuchtigkeitskontaminiertes DMSO kann die Löslichkeit verringern. Verwenden Sie frisches, wasserfreies DMSO.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 4 mg/mL (6.58 mM)
(Feuchtigkeitskontaminiertes DMSO kann die Löslichkeit verringern. Verwenden Sie frisches, wasserfreies DMSO.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 2 mg/mL (3.29 mM)
(Feuchtigkeitskontaminiertes DMSO kann die Löslichkeit verringern. Verwenden Sie frisches, wasserfreies DMSO.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 1 mg/mL (1.64 mM)
(Feuchtigkeitskontaminiertes DMSO kann die Löslichkeit verringern. Verwenden Sie frisches, wasserfreies DMSO.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 2 mg/mL (3.29 mM)
(Feuchtigkeitskontaminiertes DMSO kann die Löslichkeit verringern. Verwenden Sie frisches, wasserfreies DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molaritätsrechner

Masse	Konzentration	Volumen	Molekulargewicht
=	×	×

Verdünnungsrechner Molekulargewichtsrechner

In vivo Charge:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Von Selleck Labs validiert. Sollten Sie Anpassungen an dieser Formulierung benötigen, kontaktieren Sie unser Verkaufsteam für kundenspezifische Tests.	0.200mg/ml (0.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In-vivo-Formulierungsrechner (Klare Lösung)

Schritt 1: Geben Sie die untenstehenden Informationen ein (Empfohlen: Ein zusätzliches Tier zur Berücksichtigung von Verlusten während des Experiments)

Dosierung: mg/kg Durchschnittsgewicht der Tiere: g Dosiervolumen pro Tier:μL Anzahl der Tiere:

Schritt 2: Geben Sie die In-vivo-Formulierung ein (Dies ist nur der Rechner, keine Formulierung. Bitte kontaktieren Sie uns zuerst, wenn es im Abschnitt "Löslichkeit" keine In-vivo-Formulierung gibt.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Berechnungsergebnisse:

Arbeitskonzentration: mg/ml;

Methode zur Herstellung der DMSO-Stammlösung: mg Wirkstoff vorgelöst in μL DMSO ( Konzentration der Stammlösung mg/mL, Bitte kontaktieren Sie uns zuerst, wenn die Konzentration die DMSO-Löslichkeit der Wirkstoffcharge überschreitet. )

Methode zur Herstellung der In-vivo-Formulierung: Nehmen Sie μL DMSO Stammlösung, dann hinzufügenμL PEG300, mischen und klären, dann hinzufügenμL Tween 80, mischen und klären, dann hinzufügen μL ddH₂O, mischen und klären.

Methode zur Herstellung der In-vivo-Formulierung: Nehmen Sie μL DMSO Stammlösung, dann hinzufügen μL Maisöl, mischen und klären.

Hinweis: 1. Bitte stellen Sie sicher, dass die Flüssigkeit klar ist, bevor Sie das nächste Lösungsmittel hinzufügen.
2. Achten Sie darauf, das/die Lösungsmittel der Reihe nach hinzuzufügen. Sie müssen sicherstellen, dass die bei der vorherigen Zugabe erhaltene Lösung eine klare Lösung ist, bevor Sie mit der Zugabe des nächsten Lösungsmittels fortfahren. Physikalische Methoden wie Vortex, Ultraschall oder ein heißes Wasserbad können zur Unterstützung des Lösens verwendet werden.

Wirkmechanismus

Targets/IC₅₀/Ki	mTORC1 (Cell-free assay) 2 nM mTOR (Cell-free assay) 4.32 nM DNA-PK (Cell-free assay) 6.34 nM mTORC2 (Cell-free assay) 10 nM p110γ (Cell-free assay) 171 nM C2α (Cell-free assay) 176 nM
In vitro	Torin1 hemmt die Phosphorylierung von mTORC1- und mTORC2-Substraten in Zellen bei Konzentrationen von 2 bzw. 10 nM. Darüber hinaus zeigt Torin1 eine 1000-fache Selektivität für mTOR gegenüber PI3K (EC50 = 1800 nM) und eine 100-fache Bindungsselektivität gegenüber 450 anderen Proteinkinasen. Torin1 verursacht einen Zellzyklusarrest über einen rapamycinresistenten Mechanismus, der auch unabhängig von mTORC2 ist. Torin1 stört mTORC1-abhängige Phänotypen vollständiger als Rapamycin. Rapamycinresistente Funktionen von mTORC1 sind für die Cap-abhängige Translation erforderlich. In einer kürzlich durchgeführten Studie wird berichtet, dass Torin1 die Neurotensinsekretion und Genexpression durch Aktivierung des MEK/ERK/c-Jun-Signalwegs in der menschlichen endokrinen Zelllinie BON erhöht.
Kinase-Assay	mTORC1- und mTORC2-in-vitro-Kinase-Assays
Kinase-Assay	Zur Herstellung von löslichem mTORC1 werden HEK-293T-Zelllinien, die N-terminal FLAG-markiertes Raptor stabil exprimieren, unter Verwendung von vesikulärem Stomatitis-Virus G-pseudotypisiertem MSCV-Retrovirus generiert. Für mTORC2 werden ähnliche HeLa-Zellen, die N-terminal FLAG-markiertes Protor-1 stabil exprimieren, generiert. Beide Komplexe werden durch Lysieren von Zellen in 50 mm HEPES, pH 7,4, 10 mm Natriumpyrophosphat, 10 mm Natrium-β-glycerophosphat, 100 mm NaCl, 2 mm EDTA, 0,3 % CHAPS gereinigt. Die Zellen werden 30 min bei 4 °C lysiert, und die unlösliche Fraktion wird durch Mikrozentrifugation bei 13.000 U/min für 10 min entfernt. Die Überstände werden 1 h lang mit FLAG-M2-monoklonalem Antikörper-Agarose inkubiert und dann dreimal mit Lyse-Puffer und einmal mit Lyse-Puffer, der eine Endkonzentration von 0,5 M NaCl enthält, gewaschen. Gereinigtes mTORC1 wird mit 100 μg/mL 3×FLAG-Peptid in 50 mm HEPES, pH 7,4, 100 mm NaCl eluiert. Das Eluat kann aliquotiert und bei -80 °C gelagert werden. Die Substrate S6K1 und Akt1 werden gereinigt. Kinase-Assays werden 20 min bei 30 °C in einem Endvolumen von 20 μL durchgeführt, bestehend aus Kinase-Puffer (25 mm HEPES, pH 7,4, 50 mm KCl, 10 mm MgCl₂, 500 μm ATP) und 150 ng inaktivem S6K1 oder Akt1 als Substrate. Die Reaktionen werden durch Zugabe von 80 μL Probenpuffer gestoppt und 5 min gekocht. Die Proben werden anschließend mittels SDS-PAGE und Immunoblotting analysiert.
In vivo	Torin1 ist in einer Dosis von 20 mg/kg in einem U87MG-Xenograft-Modell wirksam und zeigt eine gute pharmakodynamische Hemmung der nachgeschalteten Effektoren von mTOR in Tumor- und peripheren Geweben.
Literatur	[1] https://pubmed.ncbi.nlm.nih.gov/19150980/ [2] https://pubmed.ncbi.nlm.nih.gov/20860370/ [3] https://pubmed.ncbi.nlm.nih.gov/21508335/ [4] https://pubmed.ncbi.nlm.nih.gov/20508131/

Anwendungen

Methoden	Biomarker	PMID
Western blot	Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b	28757207
Immunofluorescence	lipin-1	28347999
Growth inhibition assay	Cell viability	28757207

Technischer Support

Handhabungshinweise

Tel: +1-832-582-8158 Ext:3

Wenn Sie weitere Fragen haben, hinterlassen Sie bitte eine Nachricht.

Häufig gestellte Fragen

Frage 1:
How to re-constitute it for i.p. administration?

Antwort:
It can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

C1=C0/X	C1:	LOG(C1):
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C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):